The red light responses were reversible by far-red light, indicating the presence of a photoreversible pigment.The phytochrome pigment was identified using a in 1959 by biophysicist and biochemist. Since the mutant for phytochrome B exhibited a response similar to that of the wild-type, it had been concluded that phytochrome B is not needed for low or high fluence exposure enhancement. Since the threshold of the mutant occurs at high fluence values it has been determined that phytochrome A is not responsible for curvature at high fluence values. This is because for phyA-101 the threshold for curvature occurred at higher fluences, but curvature also occurs at low fluence values. They determined that the response occurs at low fluences, and at high fluences.
Thus leading to the conclusion that two phases must be responsible for phototropism. The phytochrome expressing more than normal amounts of phytochrome B exhibited curvatures similar to that of the wild type at different fluences of red light up until the fluence of 100umol-m −2 at fluences higher than this curvature was much higher than the wild-type.Thus, the experiment resulted in the finding that another phytochrome than just phytochrome A acts in influencing the curvature since the mutant is not that far off from the wild-type, and phyA is not expressed at all.
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